Resumen
- The transmission electron microscopy (TEM) was performed to study the ultrastructure of ovarian follicles that were considered morphologically normal and viable of the non-cultured (control) and cultured cells for seven days with minimum essential medium alpha modified (α-MEM) and tissue medium culture 199 (TCM-199) supplemented (+) with 20 mM HEPES; 3 mM glutamine; 10 ng/mL insulin; 2.5 g/mL transferrin; 4 ng/mL selenium; 0.1% bovine serum albumin; 50 mg/mL ascorbic acid (α-MEM except); 0.1 mg/mL penicillin and 0.1 mg/mL streptomycin. Ovarian cortical tissues (n = 12) from crossbred cows at different ages and phases of the estrous cycle were collected from a local slaughterhouse.