Importance of dna quality in the sensitivity of detection of phytophthora ramorum Academic Article uri icon

Resumen

  • Phytophthora ramorum, the causal agent of Sudden Oak Death (SOD) is a quarantine pathogen that is requiring the scrutiny of thousands of samples to track the movement of infected material. Under federal mandate, USDA APHIS has issued specific guidelines for pathogen detection and has implemented strict control points that guarantee the proficiency of the employed tests. However, the accuracy of the results can be influenced by the quality of the DNA template used for PCR amplification