The objective of this study was to select a wild yeast strain witha good performance in very high gravity (VHG) fermentation. Anumber of yeast strains were isolated from a fermented sugar canejuice and sugar cane cutting mill in an ethanol production com-pany. Interdelta analysis with primer pair (delta12-delta21) wasdone to confirm the differences between the isolated strains pre-viously identified asSaccharomyces cerevisiaewith ITS method.VHG fermentation performance was evaluated using YPD modifiedmedium with four different initial glucose concentration: 180 g/L,250 g/L, 300 g/L and 350 g/L. Ethanol Red Fermentis®, a VHG com-mercial yeast was used as a control. Specific growth rate, ethanolconcentration, yield and productivity were determined. Wild yeaststrain, 119-3, was the most promising with 0.305±0.009 h−1spe-cific growth rate and 31.53±0.07 g/L ethanol concentration witha corresponding yield and productivity of 0.09 g ethanol/g glucoseand 4.50 g/L/h, respectively
