Resumen
- The mammalian ovaries contain thousands of oocytes, most being enclosed in preantral follicles (PF), and only a small proportion will ovulate. Therefore, the biotech manipulation of oocytes enclosed in preantral follicles (MOEPF) is necessary to rescue PF of the ovarian environment (1), their cryopreservation (2) and/or in vitro culture until the maturation (3), preventing the follicular death which normally occurs in vivo. Cell damage may occur during ovarian cryopreservation procedure, mostly toxic and osmotic damages (4) caused by reactive oxygen species (ROS). Ascorbic acid (AA) is a hydrosoluble vitamin that converts ROS in inactive forms (5), it is found in ovary, granulose cells, internal teca cells and oocytes