Resumen
- Background: The composition and activity of the microorganisms are responsible for metabolic barrier functions and interactions with the host. Here, the lactic acid bacteria such as Lactobacillus casei subsp. rhamnosus have been used to produce a variety of fermented products and its metabolites lead to obtain high quality food, less perishable and organoleptic characteristics own, contributing to possible nutritional health benefits such as improved digestion of lactose. Objectives: Optimize substrate whey powder and brown sugar syrup for the production of biomass of Lactobacillus casei subsp. rhamnosus using multiple answers. Methods: a substrate honey brown sugar and whey powder was optimized through the response surface method and multiple responses, assessing biomass production, global acceptance (sensory analysis) and economic analysis. Results: a final optimal medium composed 131,284g/L whey 7,485g/L of honey and brown sugar 9,36g/L of calcium carbonate was obtained. This means presented a biomass 2,91x109 bact/mL, rating 0,57en sensory analysis and cost $1,427.85/L substrate. Conclusions: Multiple Response Optimization allowed to find predicted values for the response variable biomass production with an error of less than 8% compared to the experimental. © 2016, Universidad de Antioquia. All rights reserved