he use of plant products is a promising strategy for controlling insect pests. Therefore, extracts from six plant species were evaluated against two-spotted spider mite, Tetranychus urticae under laboratory conditions, which is a major pest of commercial rose crops in Colombia. Ethanol extracts obtained from dried leaves of Solanum nigrum, Piper elongatum, Piper aduncum, Jacaranda obtusifolia, Jatropha curcas and dried bark of Ocotea debilis were chemically characterized by means of liquid chromatography coupled to mass spectrometry. Effect of ethanolic extracts (600 µg/mL) was evaluated by direct application on eggs, larvae, and nymphs and adults of T. urticae. The mortality per stage of T. urticae was recorded after application of ethanolo extract at 168 h (eggs), 48 h (larvae), and 96 h (nymphs and adults), respectively. S. nigrum extract was the best for inducing mortality, while P. elongatum extracts reduced the fecundity of the mites. The main secondary metabolites identified in S. nigrum and P. elongatum were flavonoids, lignans, amides, and triterpene-type of compounds with potential acaricidal effects. This work constitutes the first report of the acaricidal activity of these plants against several stages and fecundity of T. urticae.