Resumen
- Background: Dental caries is a complex infectious disease of multifactorial origin in which interactions occur between plaque, tooth, biological determinants such as salivary flow, buffering capacity and pH of saliva, predominant organisms, diet and behavioral socioeconomic factors; prevails in the 60-90% of the world’s school-age population. The existing prevention and treatment are not completely effective and generate some side effects, so the search for complementary strategies is necessary for handling. Objetives: To evaluate the capability of essential oils on Lippia alba (Mill). N.E.Br and Cymbopogon citratus (DC.) Stapf to eradicate S. mutans biofilms and its toxicity on eukaryotic cells. Methods: Essential oils were extracted from plant material through steam distillation. Its chemical composition was determined for gas chromatography with mass selective detector (GC-MS). It was used the MBEC-high-throughput technique to determine the removal concentration of S. mutans biofilms. Cytotoxicity was evaluated on CHO cells through The MTT 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium.